Summary
The aim of my project is to investigate the role of lamin proteins in cancer metastasis, particularly how they influence cellular mechanics, nuclear integrity, and gene regulation during tumor progression. This research seeks to uncover potential therapeutic targets that could inhibit metastasis and improve cancer treatment outcomes.
My hypothesis is that alterations in lamin proteins contribute to enhanced metastatic potential by disrupting nuclear stability and regulating key genes involved in cancer cell migration and invasion. Understanding these mechanisms may reveal novel pathways critical to metastatic progression.
To test this hypothesis, I will analyze cancer cells with varying levels of lamin expression using molecular techniques such as Western blotting, immunofluorescence, and RNA analysis. I will examine how changes in lamin proteins impact cellular structure, nuclear morphology, and gene expression. Additionally, I will investigate the effects of lamin alterations on cancer cell migration and invasion using in vitro assays, aiming to link these findings to metastasis-related mechanisms.